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To make sure that the end of the PTFE tubing that will sit inside your hotend is square and flat, cut a slice off with a very sharp knife (a craft knife with razor sharp blade is recommended for this).

Insert the PTFE tubing. The collet clip should pre-tension the tubing and lock it into the hotend; the tubing should be held securely downwards without any ability to wiggle or move.

To release, remove the collet clip, and push down on the collet while pulling the tubing.

The PTFE lining in a Lite6 HotEnd is very important for printing successfully. Make sure that your tubing is all the way in the hotend and butting up against your nozzle (this is different from a V6, where the tubing stops above the heat break).